Union officials and farmers took the campaign to the parliament building in Edinburgh on 11 January after sending all MSPs a letter featuring statistics about prices farmers receive.
The statistics showed one in four Scottish dairy farmers has gone out of business in the past four years and Scottish farmers lose 4p/litre yet British supermarkets make around 10p/litre profit on every litre sold, the petition points out.
It also states that in 2006 a kilogram of new potatoes sold for £1.80 in Scottish supermarkets but farmers received only 17p/kg.
The letter prompted a cross-party response of more than 50 MSPs to add their names to the petition with many more who were unable to attend, also pledging their support.
Anna Davies, NFU Scotland communications and campaigns manager, told Farmers Weekly that the event had been a huge success, with many MSPs and researchers taking time for in-depth briefings on the industry.